Comparison

Enhanced superovulation

14 Item(s)

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Name PDF Type Clone Specific against Appl. Host Item no. Amount Price
CARD HyperOva Download instruction sheet Bio-active substances Other KYD-010-EX

Cosmobio
1 ml Login
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CARD HyperOva Download instruction sheet Bio-active substances Other KYD-010-06-EX

Cosmobio
600 ul Login
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CARD HyperOva Download instruction sheet Bio-active substances Other KYD-010-EX-X5

Cosmobio
5 x 1 ml Login
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CARD HyperOva Download instruction sheet Bio-active substances Other KYD-010-06-EX-X5

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5 x 600 ul Login
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CARD HyperOva (Superovulation Reagent for mouse) Download instruction sheet Bio-active substances Mu KYD-010-06-EX-X25

Cosmobio
25 x 600 ul Login
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CARD HyperOva (Superovulation Reagent for mouse) Download instruction sheet Bio-active substances Mu KYD-010-06-EX-X50

Cosmobio
50 x 600 ul Login
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CARD HyperOva (Superovulation Reagent for mouse) Download instruction sheet Bio-active substances Mu KYD-010-EX-X25

Cosmobio
25 x 1 ml Login
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CARD HyperOva (Superovulation Reagent for mouse) Download instruction sheet Bio-active substances Mu KYD-010-EX-X50

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CARD HyperOva FD Download instruction sheet Bio-active substances Other KYD-015-EX

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CARD HyperOva FD Download instruction sheet Bio-active substances Other KYD-015-EX-X5

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5 x 2 ml Login
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CARD HyperOva FD Download instruction sheet Bio-active substances Other KYD-015-EX-X3

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CARD HyperOva FD Download instruction sheet Bio-active substances Other KYD-015-EX-X4

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CARD HyperOva FD Bio-active substances Other KYD-015-EX-X25

Cosmobio
25 x 2 ml Login
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CARD HyperOva FD Bio-active substances Other KYD-015-EX-X50

Cosmobio
50 x 2 ml Login
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Name Price
CARD HyperOva Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 1 ml
Available
CARD HyperOva Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 600 ul
Available
CARD HyperOva Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 5 x 1 ml
Available
CARD HyperOva Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 5 x 600 ul
Available
CARD HyperOva (Superovulation Reagent for mouse) Login
Type Bio-active substances
Clone
Specific against Mu
Appl.
Host
Item no.
Amount 25 x 600 ul
Available
CARD HyperOva (Superovulation Reagent for mouse) Login
Type Bio-active substances
Clone
Specific against Mu
Appl.
Host
Item no.
Amount 50 x 600 ul
Available
CARD HyperOva (Superovulation Reagent for mouse) Login
Type Bio-active substances
Clone
Specific against Mu
Appl.
Host
Item no.
Amount 25 x 1 ml
Available
CARD HyperOva (Superovulation Reagent for mouse) Login
Type Bio-active substances
Clone
Specific against Mu
Appl.
Host
Item no.
Amount 50 x 1 ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 1 x 2 ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 5 x 2 ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 3x2ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 4x2ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 25 x 2 ml
Available
CARD HyperOva FD Login
Type Bio-active substances
Clone
Specific against Other
Appl.
Host
Item no.
Amount 50 x 2 ml
Available

14 Item(s)

per page

CARD HyperOva®

Great for IVF

Use CARD HyperOva® to obtain more ovulated oocytes.

PRODUCT OUTLINE

CARD HyperOva® (Japanese Patent No. 5927588) is an ultra-superovulation reagent that induces ovulation at higher efficiency when compared with the conventional superovulation induction method (PMSG-hCG method). Following the conventional method, we were only able to obtain approximaely 20 ova per mouse (C57BL/6J) on average; using CARD HyperOva® however, we can obtain as many as 3-4 times more ova.

EFFECTS

   Redction in Experimental Animal Use (ibution to 3R):

Using CARD HyperOva® enable us to reduce the number of female mice for ovum collection by 25 – 30 %.

   Optimization of Genetically Modified mouse Certion and Preservation:

More ova can be obtained from less female mice using CARD HyperOva® than following the conventional method, wich will facilitate in vitro fertilization and embrio transfare.

   Application to Valuable Genetically modified Mice:

Using CARD HyperOva® on your valuable genetically modified mice enables you to obtain a lot of ova from small number of females, thus enabling the large-scale preparation of embryos

 

SUPEROVULATION PROZEDURE:

  1. Inject 0.1-0.2 mL CARD HyperOva® i.p. into a 26-30 days old female mouse (birthsdate= 0). Injections are usually performed during the light cycle, between 17:00 - 18:00.
  2. At 48 hours after CARD HyperOva® recipients are injected i.p. with 7,5 IU human chorionic gonadotropin (hCG, not included).

No.

Stains

Method

Number of oocyte donors

Number of inseminated oocytes

Average number of ovulated oocytes / female

No. of fertilized eggs

Fertilization rate in %

1

C57BL/6J

eCG

10

277

27,7 ± 5,4

267

96,4 ± 3,2

Produkt

10

1072

107,2 ± 22,7

936

89,8 ± 3,7

2

BALB/c ByJ

eCG

10

293

29,3 ± 8,9

213

73,4 ± 11,3

Produkt

10

903

90,3 ± 14,5

680

76,4 ± 9,0

3

C3H/HeJ

eCG

10

286

28,6 ± 8,4

251

87,8 ± 22,6

Produkt

10

520

52,0 ± 18,1

446

85,8 ± 15,5

4

DBA/2J

eCG

10

225

22,5 ± 7,8

194

86,2 ± 11,0

Produkt

10

688

68,8 ± 13,6

592

86,0 ± 4,5

5

FVB/NJ

eCG

10

168

16,8 ± 3,3

159

94,6 ± 6,7

Produkt

10

256

25,6 ± 5,9

239

93,3 ± 6,6

6

CD1

eCG

10

202

20,2 ± 8,0

146

72,3 ± 16,0

Produkt

10

337

33,7 ± 9,9

269

79,8 ± 19,6

 

Figure: (A) In conventional superovulation treatment, the number of oocytes increases by exogenous injection of eCG.
(B) By injecting anti-inhibin serum (AIS), endogenous inhibin secretion is neutralized and endogenous FSH secretion is maintained at a high level. This can induce a significantly larger number of developing follicles. (see reference 3.)

 

Referenzen:

  1. Takeo T., Nakagata N. 2015. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS ONE 10(5): e0128330. doi:10.1371/journal.pone.0128330
  2. Takeo T., Nakagata N. 2016. Immunotherapy using inhibin antiserum enhanced the efficacy of equine chorionic gonadotropin on superovulation in major inbred and outbred mice strains. Theriogenol. doi:10.1016/j.theriogenology.2016.04.076
  3. Mochida, K., Hasegawa, A., & Ogura, A. 2017. Recent technical breakthroughs for ARTs in mice. Journal of Mammalian Ova Research, 34(1), 13-21.

Description

Cat. Nr.

Quantity

Storage

CARD HyperOva®

KYD-010- EX

1 mL

-20°C

KYD-010-EX-X5

5 x 1 mL

-20°C

Shipping: Dry Ice