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ELISA
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| Human Autoimmune Disease IgG Autoantibody Array G1 | Login | Human Autoimmune Disease IgG Autoantibody Array G1 | ||||||||||||||||
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| Recombinant Human ACY1 T7 | Login | Recombinant Human ACY1 T7 | ||||||||||||||||
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| Recombinant Human AGRP/Agouti | Login | Recombinant Human AGRP/Agouti | ||||||||||||||||
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| Recombinant Human Angiopoietin-like 3 Protein His | Login | Recombinant Human Angiopoietin-like 3 Protein His | ||||||||||||||||
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| Recombinant Human Angiopoietin-like 4 Protein His | Login | Recombinant Human Angiopoietin-like 4 Protein His | ||||||||||||||||
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| Recombinant Human Annexin 7 His | Login | Recombinant Human Annexin 7 His | ||||||||||||||||
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| Synthetic Avian Influenza A H5N1 HA (NT) Blocking Peptide | Login | Synthetic Avian Influenza A H5N1 HA (NT) Blocking Peptide | ||||||||||||||||
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| Trout C1q-LP3 ELISA kit, 1 x 96 wells | Login | Trout C1q-LP3 ELISA kit, 1 x 96 wells | ||||||||||||||||
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| Trout Carbonic Anhydrase (CA1) ELISA | Login | Trout Carbonic Anhydrase (CA1) ELISA | ||||||||||||||||
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| Trout Cathelicidin ELISA kit, 1 x 96 wells | Login | Trout Cathelicidin ELISA kit, 1 x 96 wells | ||||||||||||||||
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| Trout Compliment Factor H Like 1 (CFHL-1) ELISA | Login | Trout Compliment Factor H Like 1 (CFHL-1) ELISA | ||||||||||||||||
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| Trout Complement Factor H Like (CFHL-1) ELISA | Login | Trout Complement Factor H Like (CFHL-1) ELISA | ||||||||||||||||
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| HUMAN SAA ELISA | Login | HUMAN SAA ELISA | ||||||||||||||||
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| Human NE | Login | Human NE | ||||||||||||||||
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| Chicken Ex-FABP | Login | Chicken Ex-FABP | ||||||||||||||||
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| Chicken Hemopexin | Login | Chicken Hemopexin | ||||||||||||||||
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| Dog IgA | Login | Dog IgA | ||||||||||||||||
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| Dog NGAL | Login | Dog NGAL | ||||||||||||||||
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| Dog PAP-1 | Login | Dog PAP-1 | ||||||||||||||||
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| Cow (milk) SAA3 | Login | Cow (milk) SAA3 | ||||||||||||||||
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| Rabbit C-Reactive Protein (CRP) ELISA, new version, 1 x 96 wells | Login | Rabbit C-Reactive Protein (CRP) ELISA, new version, 1 x 96 wells | ||||||||||||||||
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| Trout Fibrinogen ELISA | Login | Trout Fibrinogen ELISA | ||||||||||||||||
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| Trout Growth Differentiation Factor-15 (GDF-15) ELISA | Login | Trout Growth Differentiation Factor-15 (GDF-15) ELISA | ||||||||||||||||
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| Trout Haptoglobin ELISA kit, 1 x 96 wells | Login | Trout Haptoglobin ELISA kit, 1 x 96 wells | ||||||||||||||||
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| Shrimp Heat Shock Protein 40 (HSP40) ELISA | Login | Shrimp Heat Shock Protein 40 (HSP40) ELISA | ||||||||||||||||
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Enzyme Linked Immunosorbent Assay.
For the detection of proteins, viruses or low molecular weight compounds (for example: hormones, pesticides and toxins) and their concentration determination the Enzyme Linked Immunosorbent Assay is used.
n this enzyme immunoassays (EIA) a polystyrene multiwell plate is coated with a specific antibody (capture antibody) against the sample (analyte or antigens). This immobilized antibody binds a specific amino acid sequence (epitope) on the analyte. This is followed by incubation with a second antibody (detection antibody) which can be both monoclonal origin (from hybridoma cells) as well as polyclonal (from animal serum). If these antibodies are already conjugated to a fluorophore or an enzyme, the detection can be done directly. In the event that the second antibody is uncoupled a further conjugated antibody is necessary for the detection (indirect ELISA). Thus, a characteristic color change after the addition of a substrate or a monochromatic excitation (in the case of a fluorophore-conjugated antibody) can be achieved.
The assay formats can also be divided into direct and indirect ELISA test, sandwich ELISA test and competitive or inhibitory ELISA test.
An ELISA kit offers you additional flexibility, by the ability to coat the polystyrene multiwell plate on your own.
Descriptive information about ELISA is available in our Data Library.
