Comparison

RIPA Lysis Buffer (Strong) European Partner

Manufacturer Abbexa
Category
Type Western Blot
Specific against other
Applications WB
Amount 100 ml
Item no. abx090624
eClass 6.1 32160405
eClass 9.0 32160405
Available
Description
Radioimmunoprecipitation Assay (RIPA) buffer is a lysis buffer used to lyse cells and tissue, for Western Blot and Immunoprecipitation. This buffer is more denaturing than NP-40 or Triton X-100 lysis buffer because it contains the ionic detergents SDS and sodium deoxycholate as active constituents, and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

The concentration of the protein in the lysed pellet can be determined by our BCA protein assay kits (abx090640 and abx090642). It is not recommended to use a Bradford Protein Assay Kit with this buffer.
Storage
Store at 4 C for up to one year.
Buffer
Contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin).
Dry Ice
No
Note
This product is for research use only.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ml
Available: In stock
available

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