Tissue Mitochondria Isolation Kit

Reagents and Tools

Abbexa’s Tissue Mitochondria Isolation Kit is a quick and convenient method for isolation of mitochondria from animal tissues. By isolating the mitochondria, non-mitochondrial cytoplasmic proteins are also obtained and can be used in further downstream applications. The purity of the obtained mitochondria is high, and the mitochondria often has the inner and outer membrane intact and is physiologically functional. After lysing with Mitochondria Lysis Buffer, the mitochondria can be used for SDS-PAGE, Western blot, Dimensional electrophoresis and Immunoprecipitation. One kit is sufficient for 50-100 assays if the weight per tissue is 50-100 mg.Kit Contents:

• Reagent A (Mitochondria Isolation Reagent): 60 ml
• Reagent B (Mitochondria Lysis Buffer): 20 ml
• 100X Enzyme Inhibitor: 1 ml

The exact formulation is proprietary.