Green miRNA Two-Step qRT-PCR SuperMix

Green miRNA Two-Step qRT-PCR SuperMix

Green miRNA Two-Step qRT-PCR SuperMix contains all the necessary components for the detection and quantification of miRNA from total RNA, small RNA and other miRNA-containing samples. The kit includes miRNA Enzyme Mix, containing Poly(A) polymerase and reverse transcriptase, and miRNA Reaction Mix to efficiently add Poly(A) tails and synthesize first-strand cDNA. Green qPCR SuperMix is provided for miRNA quantification. The Passive reference dye, which can be used to normalize fluorescent signals between ractions, is compatible with different qPCR instruments:

• 50X Passive Reference Dye 1:ABI Prism® 7000/7300/7700/7900, ABI Step One Plus®
• 50X Passive Reference Dye 2: ABI Prism® 7500/7500 Fast, ABI Q6, ABI QuantStudio® 6/7 Flex, ABI ViiA® 7, Stratagene Mx3000® /Mx3005P® , Qiagen Corbett Rotor-Gene® 3000.
• No Passive Reference Dye: Roche LightCycler® 480/96, MJ Research Chromo4® , MJ Research Opticon® 2, Takara TP-800® , Bio-Rad iCycler iQ® , Bio-Rad iCycler iQ5® , Bio-Rad CFX96® , Bio-Rad CI000® Thermal Cycler, Thermo Scientific Pikoreal® 96, Qiagen Corbett Rotor-Gene® 6000/G/Q

Not applicable.