Bovine Spondin-1 (SPON1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Bovine Spondin-1 microplate
Bovine Spondin-1 standard
Bovine Spondin-1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Spondin-1 is a protein contains 807 aminoacids and is structurally composed of six thrombospondin domains, one reelin domain, and one spondin domain.The deduced 624-amino acid partial SPON1 protein shares 96.8% amino acid sequence identity with the rat F-spondin precursor across 624 residues.SPON1 expression in lung, lower expression in brain, heart, kidney, liver, and testis, and lowest expression in pancreas, skeletal muscle, and ovary; no expression was found in spleen. In vitro binding assays using mutated human proteins confirmed that SPON1 specifically bound to the central APP domain (CAPPD). SPON1 inhibited APP cleavage by BACE1, the primary beta-secretase involved in APP processing. Binding also impaired APP- and FE65-dependent transactivation of the chromosome remodeling factor TIP60 .

SPON1; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein