Chicken N (6)-adenine-specific DNA methyltransferase 2 (N6AMT2) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Chicken N microplate
Chicken N standard
Chicken N detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

N6AMT2, Belongs to the methyltransferase superfamily. AML1 family. N6AMT2is a 214 amino acid protein that is encoded by a gene located on human chromosome 13q12.11. A methyltransferase is a type of transferase enzyme that transfers a methyl group from a donor to an acceptor. Methytransferases use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence, an example of epigenetic modification. This methylation occurs on cytosine residues. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice, they caused a recessive lethal phenotype.

N6AMT2; ESP13; OTTHUMP00000018103