Comparison

EliKine Progesterone ELISA Kit

Item no. ABK-KET0002-96Tx5
Manufacturer Abbkine Scientific
Amount 96Tx5
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
EliKine Progesterone ELISA Kit employs the competitive inhibition enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for progesterone and Horseradish Peroxidase (HRP) conjugated progesterone. The competitive inhibition reaction is launched between with HRP labeled progesterone and unlabeled progesterone with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of progesterone in the sample. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Serum#Plasma#Other biological fluids
Assay type
Competitive ELISA (quantitative)
Assay duration
Multiple steps standard competitive ELISA assay with a working time of 3-4 hours. It depends on the experience of the operation person.
Kit components

Progesterone microplate
Progesterone standard
HRP conjugated progesterone
Progesterone detect antibody
HRP substrate A
HRP substrate B
Stop solution
Wash buffer
Plate covers.
Features & Benefits
EliKine Progesterone ELISA Kit has high sensitivity and excellent specificity for detection of progesterone. No significant cross-reactivity or interference between progesterone and analogues was observed.
Calibration range
0.5 ng/ml-30 ng/ml
Limit of detection
0.2 ng/ml
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for
Storage instructions
The unopened kit should be stored at 2 - 8 C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Progesterone is an endogenous steroid and progestogen sex hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. It belongs to a group of steroid hormones called the progestogens, and is the major progestogen in the body. Progesterone is also a crucial metabolic intermediate in the production of other endogenous steroids, including the sex hormones and the corticosteroids, and plays an important role in brain function as a neurosteroid.
Alternative
pregn-4-ene-3, 20-dione; P4

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx5
Available: In stock
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