Comparison

Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit

Item no. ABK-KTE60308-96T
Manufacturer Abbkine Scientific
Amount 96T
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF18 in samples. An antibody specific for TM4SF18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Transmembrane 4 L6 family member 18 microplate
Human Transmembrane 4 L6 family member 18 standard
Human Transmembrane 4 L6 family member 18 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Transmembrane 4 L6 family member 18 (TM4SF18) ELISA Kit has high sensitivity and excellent specificity for detection of Human TM4SF18. No significant cross-reactivity or interference between Human TM4SF18 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TM4SF18 also known as AAB5 and L6D, was cloned from human umbilical vein endothelial cells. It shares approximately 40 - 50% amino acid sequence identity with L6, IL-TMP, and TM4SF5, a group of proteins with limited homology to the tetraspanin superfamily.
TM4SF18 belongs to the L6 tetraspanin family, L6, IL-TMP, and TM4SF5 are cell surface proteins predicted to have four transmembrane domains. Previous sequence analysis led to their assignment as members of the tetraspanin superfamily.A new sequence (L6D) that is strikingly similar to L6, IL-TMP, and TM4SF5. Analyses of these four sequences indicate that they are not significantly related to genuine tetraspanins, but instead constitute their own L6 superfamily.
Gene ID
116441
Alternative
TM4SF18; L6D;
Accession
Q96CE8

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96T
Available: In stock
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