« Back
Home /
Human Toll-like receptor 7 (TLR-7) ELISA Kit

Human Toll-like receptor 7 (TLR-7) ELISA Kit

Item no.	ABK-KTE62321-96T
Manufacturer	Abbkine Scientific
Amount	96 T
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	TLR7
Available
Manufacturer - Applications	This Human Toll-like receptor 7 (TLR-7) ELISA Kit employs a two-site sandwich ELISA to quantitate TLR7 in samples. An antibody specific for TLR7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTLR7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Background	Toll-like receptor 7is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. Imiquimod acts upon TLR 7. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Toll-like receptor 7 (TLR-7) ELISA Kit has high sensitivity and excellent specificity for detection of Human TLR7. No significant cross-reactivity or interference between Human TLR7 and analogues was observed.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	Human Toll-like receptor 7 microplate Human Toll-like receptor 7 standard Human Toll-like receptor 7 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 96 T

not available