Comparison

Mouse Taste receptor type 2 member 13 (TAS2R13) ELISA Kit

Item no. ABK-KTE70354-96Tx50
Manufacturer Abbkine Scientific
Amount 96Tx50
Category
Type Elisa-Kit
Applications ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Mouse Taste receptor type 2 member 13 (TAS2R13) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R13 in samples. An antibody specific for TAS2R13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Taste receptor type 2 member 13 microplate
Mouse Taste receptor type 2 member 13 standard
Mouse Taste receptor type 2 member 13 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Taste receptor type 2 member 13 (TAS2R13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAS2R13. No significant cross-reactivity or interference between Mouse TAS2R13 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TAS2R13 product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. TAS2R13 maps to the taste receptor gene cluster on chromosome 12p13.
Gene ID
387349
Alternative
TAS2R13; T2R13; TRB3; taste receptor; family B; member 3
Accession
Q7M720

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96Tx50
Available: In stock
available

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