Comparison

Rat Acid phosphatase-like protein 2 (ACPL2) ELISA Kit

Item no. ABK-KTE100327-48T
Manufacturer Abbkine Scientific
Amount 48T
Category
Type Elisa-Kit
Applications ELISA
Specific against Rat (Rattus norvegicus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Available
Applications notes
This Rat Acid phosphatase-like protein 2 (ACPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate ACPL2 in samples. An antibody specific for ACPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Rat Acid phosphatase-like protein 2 microplate
Rat Acid phosphatase-like protein 2 standard
Rat Acid phosphatase-like protein 2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Rat Acid phosphatase-like protein 2 (ACPL2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ACPL2. No significant cross-reactivity or interference between Rat ACPL2 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
ACPL2 belongs to the histidine acid phosphatase family. Catalyses the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.
Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase. It is stored in lysosomes and functions when these fuse with endosomes, which are acidified while they function; therefore, it has an acid pH optimum. Different forms of acid phosphatase are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. For example, elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer.
Gene ID
315939
Alternative
ACPL2; FLJ23751;
Accession
Q66H78

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 48T
Available: In stock
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