PurKine His-Tag Protein Purification Kit (Ni-NTA)

PurKine His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90um beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

High capacity—More than 50 mg of 6xHis-tagged protein per milliliter of resin.

Versatile—For purification under native or denaturing conditions.

Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.

Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Easy to use— Pre-formulated buffers available for kit formats.

Blue ice

PurKine His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine His-Tag Purification resin is also available as prepacked spin column and kit formats.