Human IL-1 beta protein

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 ug/mL (100ul/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-8 pg/mL.
3. Measured by its binding ability in a functional ELISA.
4. Immobilized human IL1B at 10 ug/mL (100 ul/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 ug/mL.
5. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2 ng/mL.

E. coli

Lyophilized from sterile PBS, pH 7.4

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1 beta protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The product is shipped at ambient temperature.

Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc).

IL-1; IL-1 beta; IL-1B; IL1-BETA; IL1F2; Interleukin-1 beta; IL1B