Chemiluminescent Substrate Solution (AE Marker)

Buffer

RT

The chemiluminescence substrate solution is a simple triggering reagent for acridinium ester (AE) fast light emission. Acridinium ester is the most commonly used for protein labeling and widely used in automated instruments because of its high sensitivity with detection limits in the attomole range. This product is suitable for acridinium ester labels on chemiluminescence technology, it is provided in two liquid buffer of Trigger A (Oxidant solution) and Trigger B (Enhancer solution) in each kit. When used, mix Trigger A and Trigger B in equal volume by 1:1, and light emission from acridinium esters is quite rapid and is essentially complete in <5s.