DMEM High Glucose

3821000000

Ambient

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Dulbecco's Modified Eagle Medium (DMEM) is modified to contain four times the amino acid and vitamin concentration of original Eagle's Minimal Essential Medium. The original DMEM formulation contained a low concentration of glucose (1 g/L). However, this DMEM formulation is available with higher glucose levels. DMEM also uses a sodium bicarbonate buffer system, allowing for maintenance of physiological pH in a 5–10% CO2 environment. Importantly, DMEM commonly requires fetal bovine serum (FBS) supplementation.