Active Recombinant Human Activin AB protein

betaA C600H911N173O174S13/ betaB - C615H910N178O177S12

1.56

Endotoxin Level : < 0.04 EU / ug protein (LAL method)

Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGFbeta family. Mature Activin AB has two chains of 116 and 123 amino acids residues (betaA-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins /Activins are proteins that are formed by the dimerization of two subunits, i. e. an alpha with either betaA –inhibin A- or betaB - inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers calleds activins: Activin A (betaAbetaA), Activin B (betaBbetaB) and Activin AB (betaAbetaB). The activin gene family comprises the additional, but poorly characterized members activin betaC, betaD, and betaE. - As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. - The development of assays distinguishing between different forms of activins and inhibins, along with knock-in and knock-out models, have provided evidence that the betaA- and betaB-subunits have independent and separate roles physiologically. Additionally, evaluation of ligand-receptor interactions indicates significant differences in receptor affinity between activin isoforms, as well as between inhibin isoforms.

Recombinant human Activin is lyophilized from a Tris HCl 0.05M buffer at pH 7.4

Functional studies, Cell culture, Western Blot

-

-

Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/ul. Optimal concentration should be determined for specific application and cell lines.

Room temperature