« Back
Home /
SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric - 1 kit

SensoLyte® FDP Alkaline Phosphatase Assay Kit Fluorimetric - 1 kit

Item no.	AS-71109
Manufacturer	AnaSpec
Amount	1 kit
Category	Oncology Immunohistochemistry Western Blot ELISA Other Assays & Kits Other Kits By Organ Bladder Cancer Immunostaining
Type	Assay-Kit
Applications	WB, IHC, ELISA
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	Alkaline phosphatase
Shipping Condition	Dry ice
Available
Manufacturer - Applications	Enzyme activity; ELISA; Western Blotting; Immunohistochemistry (IHC)
Manufacturer - Category	Assays Kits Group 4 / Assays Kits Group 4 / Assay Kits
Manufacturer - Targets	Phosphatase
Shipping Temperature	4 °C
Storage Conditions	Store component A at -20°C. Store all other components at 4°C.
Manufacturer - Research Area	Cell Biology & Protein Analysis / Protein Assay Kits
Description	Alkaline phosphatase is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. Conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISAs, immunohistochemical techniques and Northern, Southern and Western blot analyses. SensoLyte® FDP Alkaline Phosphatase Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in solutions, in cell extracts, in live cells as well as on solid surfaces (such as PVDF membranes). The kit contains: FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation), Calibration standard, Reaction buffer, Stop buffer, An optimized ‘mix and read’ assay protocol that is compatible with HTS liquid handling instruments
Kits Components	Component A: FDP, fluorogenic alkaline phosphatase substrate: 1 vial Component B: 2X Assay buffer: 30 mL Component C: Stop solution: 30 mL Component D: 10X Lysis buffer: 50 mL Component E: Triton X-100: 500 µL Component F: DMSO: 500 µL Component G Alkaline Phosphatase Standard, Calf Intestine: 10 µg/mL, 50 µL, sterile
Absorbtion (nm)	485
Emission (nm)	528
Detection Method	Fluorescent
Detection Limit	5 pg/ml
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105331
GTIN	5400535133283

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

AS-71109-safety-datasheet.pdf