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SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays - 1 kit

SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays - 1 kit

Item no.	AS-71126
Manufacturer	AnaSpec
Amount	1 kit
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	HCV NS3/4A Protease,hepatitis C virus nonstructural protein 3/4A
Shipping Condition	Dry ice
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Assays Kits Group 2 / Assays Kits Group 2 / Assay Kits
Manufacturer - Targets	Hepatitis C Virus (HCV)
Shipping Temperature	4 °C
Storage Conditions	Store all components at -20°C. Components C and D can be stored at room temperature for convenience. Protect Components A and B from light.
Manufacturer - Research Area	Viral & bacterial Proteases / Protein Assay Kits; Virology
Description	HCV protease is identified as an important drug-screening target. The SensoLyte® 490 HCV Protease Assay Kit uses an optimized FRET peptide substrate for the continuous measurement of HCV NS3/4a activity. This FRET substrate is cleaved specifically by HCV NS3/4a protease thereby liberating the C-terminal peptide-fluorophore fragment from the proximity quenching effect of the dark quencher, resulting in a more than 10-fold increase in fluorescence. This assay can detect HCV NS3/4a protease activity at 1 pmole.
Kits Components	Component A: HCV NS3/4A protease substrate Ex/Em=340 nm/490 nm upon cleavage: 600 µL Component B: EDANS, fluorescence reference standard: 100 µM DMSO solution, 20 µL Component C: 2X Assay buffer: 50 mL Component D: Stop solution: 30 mL Component E: DTT: 1 M, 1 mL X 2 vials Component :F Pep4AK, HCV NS3 protease cofactor: 300 µL, 600 µM
Absorbtion (nm)	340
Emission (nm)	490
Detection Method	Fluorescent
Detection Limit	1 pmole
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105331
GTIN	5400535133160

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-71126-safety-datasheet.pdf