« Back
Home /
SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

SensoLyte® Plus 520 MMP-9 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

Item no.	AS-72017
Manufacturer	AnaSpec
Amount	1 kit
Category	Oncology Neuroscience Neuroinflammation By Cell Type ELISA Other Assays & Kits Other Kits Immunostaining
Type	Assay-Kit
Applications	ELISA
Specific against	other
Host	CHO cells, Mammalian cells
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	MMP-9,Matrix metalloproteinase 9,gelatinase-B,collagenase-IV
Shipping Condition	Dry ice
Available
Manufacturer - Applications	ELISA + FRET; Protease activity
Manufacturer - Category	Assays Kits Group 1 / Assays Kits Group 1 / Assay Kits
Manufacturer - Targets	Matrix Metalloproteinase (MMP), MMP-9
Shipping Temperature	Dry Ice
Storage Conditions	Store component B at -80 °C. Store all other components at -20 °C. Components D, G, H and I can be stored at room temperature for convenience.
Manufacturer - Research Area	Adhesion & Extracellular Matrix; Cancer & Apoptosis / Protein Assay Kits; Tissue Remodeling/MMPs; Oncogenesis
Description	The SensoLyte® Plus 520 MMP-9 Assay Kit is designed for specifically detecting MMP-9 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-9 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-9-induced cleavage of the FRET substrate.
Kits Components	Component A: Microplate coated with monoclonal anti human MMP-9 antibody: 12 X 8 black strips Component B: MMP-9 standard, recombinant human proMMP-9: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 5 mL Component D: 10 X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-9 substrate, 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffe:r 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Absorbtion (nm)	490
Emission (nm)	520
Detection Method	Fluorescent
Detection Limit	3 ng/ml
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105331
GTIN	5400535132583

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

AS-72017-safety-datasheet.pdf