SensoLyte® 520 MMP-14 Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Matrix Metalloproteinase (MMP), MMP-14

Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4°C for convenience.

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-14 (MT1-MMP) is the membrane bound MMP. When expressed in cells, the catalytic and the hemopexin domains of MMP-14 become exposed on cell surfaces. This enzyme plays an important role in tumor invasion. SensoLyte® 520 MMP-14 Assay Kit uses a 5-FAM (fluorophore) and QXL™520 (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon the cleavage of the FRET peptide by MMP-14, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL™520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

490

One unit of protease hydrolyzes 1 picomole of the DABCYL/EDANS substrate in SensoLyte™ 490 HIV Protease Assay Kits (AnaSpec Cat# 71127) per min at pH 4.7 at 25°C.

2 ng/ml

Research use

5400535132477