Comparison

AnaTag™ AMCA Kit - 1 kit

Item no. AS-72055
Manufacturer AnaSpec
Amount 1 kit
Category
Type Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Protein labeling kit
Shipping Condition Dry ice
Available
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye
Shipping Temperature
4 °C
Storage Conditions
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Manufacturer - Research Area
Peptide & Protein Analysis / Protein Labeling Kits
Description
AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442±10 nm when excited at 353±10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.
Note: AnaTag kits are not intended for peptide labeling.
Kits Components
Component A: AMCA-X SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 1 mL Component E: 10x Elution buffer for eluting dye-protein conjugate: 30 mL
Absorbtion (nm)
353
Emission (nm)
442
Detection Method
Fluorescent
Feature
Blue (<500 nm)
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
41105331
GTIN
5400535132071

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 kit
Available: In stock
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