Comparison

AnaTag™ APC Labeling Kit - 1 kit

Item no. AS-72111
Manufacturer AnaSpec
Amount 1 kit
Category
Type Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Protein labeling kit
Shipping Condition Dry ice
Available
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Protein
Shipping Temperature
4 °C
Storage Conditions
Store all components at 4°C. Protect component A from light and do not freeze.Components B and H may be frozen for best performance.
Manufacturer - Research Area
Peptide & Protein Analysis / Protein Labeling Kits
Description
The AnaTag™ APC Labeling Kit is optimized for use in the conjugation of allophycocyanin (APC) to antibodies. APC is an ultra-sensitive, near-infrared fluorescent tracer with a high quantum yield (Ex/Em = 650 nm/660 nm). APC- labeled antibodies are used in applications such as flow cytometry and immunofluorescence. The AnaTagTM APC Labeling Kit contains SH-reactive APC. SMCC modified allophycocyanin reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. AnaTagTM APC Labeling Kit contains a chemically cross-linked APC (CL-APC) that is much more stable than the native APC, but still retains the original spectroscopic properties. The amount of APC supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Cross-linked and SMCC-activated APC are also available as separate products. Kit Size: 1 labeling reaction (1x1 mg IgG)
Note: AnaTag kits are not intended for peptide labeling.
Kits Components
Component A: Activated CL-APC (SMCC activated cross-linked allophycocyanin): 2 mg, 10mg/mL Component B: DTT, reduces antibody: 0.1 mL Component C: Spin column which desalts protein by centrifugation: 1 column Component D: Wash tube to collect buffer from the spin column: 1 tubeComponent E: Collection tube to collect sample from the spin column: 1 tube Component F: Gravity column which desalts protein by gravity: 1 column Component G: DMSO: 1 mL Component H: NEM which blocks free sulfhydryl groups: 1 vial Component I: Reaction buffer for conjugation of APC with protein: 100 mL
Absorbtion (nm)
650
Emission (nm)
660
Detection Method
Fluorescent
Feature
Red (620 -750 nm)
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
41105331
GTIN
5400535131586

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 kit
Available: In stock
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