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SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric - 1 kit

SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric - 1 kit

Item no.	AS-72124
Manufacturer	AnaSpec
Amount	1 kit
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	Plasmin
Shipping Condition	Dry ice
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Assays Kits Group 1 / Assays Kits Group 1 / Assay Kits
Manufacturer - Targets	Plasmin
Shipping Temperature	Dry Ice
Storage Conditions	Store component C at -80°C. Store all other components at -20°C. Component D and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Manufacturer - Research Area	Cardiovascular Research / Protein Assay Kits; Coagulation
Description	Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis. The SensoLyte® AFC Plasmin Activity Assay Kit provides a convenient assay for high throughput screening of plasmin inhibitors and inducers or for continuous assay of plasmin activity. The assay utilizes a fluorogenic peptide, which upon enzyme cleavage releases the AFC (7-amido-4-trifluoromethylcoumarin) fluorophore. The fluorescent signal can be measured at Ex/ Em =380 nm/500 nm. This assay can detect picogram level of plasmin activity.
Kits Components	Component A: Plasmin substrate, Ex/Em=380 nm/500 nm upon cleavage: 3 mM, 50 µL Component B: AFC, fluorescence reference standard, Ex/Em=380 nm/500 nm: 3 mM, 10 µL Component C: Human plasmin: 250 µg/mL, 10 µL Component D: 2X Assay Buffer: 10 mL Component E: Plasmin Inhibitor: 1 mM, 10 µL Component F: Stop Solution: 5 mL
Absorbtion (nm)	380
Emission (nm)	500
Detection Method	Fluorescent
Detection Limit	0.8 ng/ml
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105331
GTIN	5400535131487

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72124-safety-datasheet.pdf