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SensoLyte® 520 Deubiquitination Assay Kit Fluorimetric - 1 kit

Item no. AS-72203
Manufacturer AnaSpec
Amount 1 kit
Category
Type Assay-Kit
Specific against other
Host E.coli
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Shipping Condition Dry ice
Available
Manufacturer - Applications
FRET; Protease activity
Manufacturer - Category
Assay Kits
Manufacturer - Targets
Deubiquitination
Manufacturer - Host
E.coli
Shipping Temperature
Dry Ice
Storage Conditions
Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Description
Deubiquitinating enzymes (DUBs) are proteases that reverse ubiquitin modifications and can rescue a protein from proteasomal degradation. DUBs specifically cleave ubiquitin-linked molecules downstream of the terminal carbonyl of the last residue of ubiquitin (Gly76). They are classified in five families. Well-defined families include ubiquitin carboxyl-terminal hydrolases (UCH) and ubiquitin-processing proteases (UBP). DUBs act as regulators of diverse cellular processes, such as development, growth and DNA repair. They have become actively studied molecular targets for drug discovery since they are implicated in the pathogenesis of many human diseases. The SensoLyte 520 Deubiquitination Assay Kit provides a convenient assay for the screening of enzyme inhibitors and activators or for continuous assay of deubiquitination activity through the use of a fluorogenic peptide substrate. Upon the cleavage by a deubiquitin protease, this substrate generates the green fluorophore that can be detected at excitation/emission=490/520 nm. The kit contains: - The 520 DUB fluorogenic substrate (Ex/Em=490/520 nm upon cleavage) - Assay buffer - Deubiquitin protease UCH-L3 - Inhibitor - Fluorescence reference standard for calibration - A detailed protocol Kit size: 100 assays (96-well plate)
Product Group
NA1ZZZ
Research Area
Epigenetics / 0
Absorbtion (nm)
490
Emission (nm)
520
GTIN
5400535130510
Detection Method
Fluorescent
Usage
Research use
UNSPSC
41105331

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 kit
Available: In stock
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