SensoLyte® Thioflavin T ß-Amyloid (1-40) Aggregation Kit - 1 kit

Aggregation/Fibrillation

beta-amyloid, beta-amyloid (1-40), Thioflavin T

Store kit components at 4°C. Components B, D, and E should be stored at -20°C if not used within one week

The SensoLyte® ThT ?-Amyloid (1-40) Aggregation kit provides a convenient and standard method to measure A?40 aggregation using Thioflavin T dye. A?40 peptide is pretreated to ensure it is in a monomeric state. An optimized fibrillation buffer is included with the kit, and two known inhibitors are supplied as controls. The assay is based on the property of ThT dye in which fluorescence (Ex/Em=440/484 nm) is increased when bound to aggregated A? peptides. Alzheimer's disease (AD), the most common cause of dementia, is characterized by the presence of senile plaques and neurofibrillary tangles, surrounded by damaged neurons. Beta-Amyloid (A?) peptides, A?40 (1-40) and A?42 (1-42), were found to be a major component of the above plaques. Many studies suggest that these peptides can form toxic oligomers and fibrils under physiological conditions and rapidly aggregate. Since A? aggregation is evidently an essential event in the pathogenesis of AD, a reliable assay is important to study A? fibrillation kinetics and screen for A? aggregation inhibitors.

440

Fluorescent

Research use

5400535130404