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SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit Fluorimetric - 1 kit

SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit Fluorimetric - 1 kit

Item no.	AS-72255
Manufacturer	AnaSpec
Amount	1 kit
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Dry ice	Yes
Citations	1. Bryant, J.W. et al, Cardiovasc Hematol Agents Med Chem 7, 234 (2009)2. Toossi, Z. et al. Proc Natl Acad Sci U S A 89, 11969 (1992)3. Ghebrehiwet, B. et al. J Exp Med 153, 665 (1981)4. Qin, X.P. et al. Clin Exp Pharmacol Physiol 36, 319 (2009)5. Phipps, J.A. et al. Hypertension 53, 175 (2009)6. DiScipio, R.G. Immunology 45, 587 (1982)7. Kaplan, A.P. et al. J Exp Med 135, 81 (1972)8. Wachtfogel, Y.T. et al. J Clin Invest 72, 1672 (1983)9. Ichinose, A. et al, J Biol Chem 261, 3486 (1986)
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Dry ice
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Assay Kits
Manufacturer - Targets	Kallikrein
Shipping Temperature	Dry Ice
Storage Conditions	Store component C at -80°C. Store all other components at -20°C. Component D and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Description	Human plasma kallikrein, a serine protease, is first synthesized as inactive plasma prekallikrein in liver and circulates in the plasma. Once activated by activated coagulation factor XII (FXIIa), plasma kallikrein releases bradykinin (an inflammatory mediator) from high molecular weight kininogen (HMWK). The SensoLyteRh110 Plasma Kallikrein Activity Assay Kit employs a fluorescence peptide substrate for the detection of human plasma Kallikrein activity. This substrate contains rhodamine 110 fluorophore (Rh110). Plasma kallikrein cleaves this Rh110 substrate and results in the release of a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components. This assay can detect as low as 1 ng/mL active plasma Kallikrein. This kit could be used to detect enzyme activity in purified enzyme preparations, biological samples and it can also be applied for screening inhibitors. Kit size: 100 assays (96-well plate)
Product Group	NA1ZZZ
Research Area	Cardiovascular Research / 0
Absorbtion (nm)	490
Emission (nm)	520
GTIN	5400535174705
Detection Method	Fluorescent
Detection Limit	1 ng/ml
Usage	Research use
UNSPSC	41105331

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72255-safety-datasheet.pdf