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SensoLyte® Rh110 Furin Activity Assay Kit Fluorimetric - 1 kit

SensoLyte® Rh110 Furin Activity Assay Kit Fluorimetric - 1 kit

Item no.	AS-72256
Manufacturer	AnaSpec
Amount	1 kit
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Host	Mammalian cells
Conjugate/Tag	Unconjugated
Dry ice	Yes
Citations	1.Van den Ouweland, A.M. et al. Nucleic Acids Res. 18, 664 (1990).2. Watanabe T. et al. J. Biol. Chem. 267:8270 (1992). 3. Molloy S.S. et al. J. Biol. Chem. 267: 16396 (1992). 4. Thomas, G. et al. Nature Rev. Mol. Cell Biol. 3:753 (2002) . 5. Dahms S. O. et al. PNAS 113:11196 (2016)
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Dry ice
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Assay Kits
Manufacturer - Targets	Furin
Manufacturer - Host	Mammalian Cells
Manufacturer - Host	NSO (mammalian cells)
Shipping Temperature	Dry Ice
Storage Conditions	Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Description	Furin is a serine protease that belongs to the subtilisin-like proprotein convertase (PC) family. Furin catalyzes the proteolytic maturation of proprotein substrates in the secretory pathway. Furin plays an essential role in embryogenesis and homeostasis, as well as in diseases ranging from Alzheimer's disease, cancer, anthrax, to Ebola fever. Furin is essential to the maturation and viral infectivity of HIV. Furin is a potential target for drug design, especially for the inhibition of furin-dependent virus replication. The SensoLyte Rh110 Furin Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of furin activity using a fluorogenic substrate. Upon cleavage by furin, this substrate generates the Rh110 (rhodamine 110) fluorophore with bright green fluorescence that can be detected at excitation/emission=490 nm/520 nm. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from other reaction components. The detection limit can reach as low as 0.02 ng/mL.
Product Group	NA1ZZZ
Research Area	Viral & bacterial Proteases; Neuroscience; Cancer & Apoptosis / 0
Absorbtion (nm)	490
Emission (nm)	520
GTIN	5400535175856
Detection Method	Fluorescent
Detection Limit	0.02 ng/ml
Usage	Research use
UNSPSC	41105331

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72256-safety-datasheet.pdf