SensoLyte® Blue Glucocerebrosidase (GBA) Activity Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Glucocerebrosidase

Store component C at -80°C. Store all other components at -20°C. Component D and F can be stored at room temperature for convenience. Protect components A and B from light and moisture.

Glucocerebrosidase/GBA (also called acid ?-glucosidase, Glucosylceramidase) is a lysosomal enzyme responsible for the breakdown of glucocerebroside releasing glucose and ceramide. Deficiency of this enzyme due to genetic mutations leads to accumulation of glucocerebroside and development of lysosomal storage disease, known as Gaucher disease (GD). Mutations in the glucocerebrosidase (GBA1) gene are also associated with increased risk for Parkinson disease and related disorders. It has been hypothesized that GBA, when not available to clear out proteins like alpha-synuclein, results in the accumulation of the proteins thereby contributing to Parkinson’s disease.
The SensoLyte® Blue Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a most common fluorogenic substrate, MUGlc, for measurement of GBA activity. In presence of GBA the colorless substrate is hydrolyzed into 4-methylumbelliferone (4MU), and after addition of stop solution a bright blue fluorescence can be monitored at excitation/emission = 365nm/445nm nm.

365

Fluorescent

Research use

5400535176334