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Bacterial Sortase Substrate I, FRET

Bacterial Sortase Substrate I, FRET

Item no.	AS-62231
Manufacturer	AnaSpec
Amount	1 mg
Category	Peptides & Proteins Peptides
Type	Peptides
Format	Lyophilized
Specific against	other
Conjugate/Tag	Biotin
Purity	Peak Area by HPLC ≥95%
Sequence	DABCYL-Leu-Pro-Glu-Thr-Gly-EDANS
Citations	Mazmanian, S. et al. PNAS 99, 2293 (2002); Ilangovan, U. et al. PNAS 98, 6056 (2001), Ton-That, H. et al. PNAS 96, 12424 (1999); W. Navarre and O. Schneewind Microbiol. Mol. Biol. Rev. 63, 174 (1999).
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	DABCYL-LPETG-EDANS
Shipping Condition	Room temperature
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Catalog Peptides / Catalog Peptides Gr / Peptides / Labeling & detection / Blue (<500 nm)
Manufacturer - Targets	Sortase A
Manufacturer - Conjugate / Tag	Double dyes: EDANS-DABCYL
Shipping Temperature	RT
Storage Conditions	- 20 °C Protected from light
Molecular Weight	1015.2
Manufacturer - Research Area	Immunology and Infectious disease; Cell Signaling / Microbiology/Bacteriology; Enzyme Substrates & Inhibitors
Description	This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.
Absorbtion (nm)	340
Emission (nm)	490
Sequence One-Letter Code	DABCYL-LPETG-EDANS
Detection Method	Fluorescent
Feature	FRET Substrates
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	12352202
GTIN	5400535149543

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AS-62231-safety-datasheet.pdf