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Protein A-HiLyte™ Fluor 555 Conjugate

Protein A-HiLyte™ Fluor 555 Conjugate

Item no.	AS-72236
Manufacturer	AnaSpec
Amount	1 mg
Category	Peptides & Proteins Recombinant Proteins Immunohistochemistry Immunoprecipitation Western Blot Immunofluorescence
Type	Proteins Recombinant
Format	Liquid
Applications	WB, IF, IP, IHC
Specific against	other
Host	E.coli
Conjugate/Tag	Unconjugated
Purity	>95% by SDS-PAGE
Dry ice	Yes
Citations	1. Björk, I. Eur J Biochem 29 (1972):579-584. 2. Sjödahl, J. Eur J Biochem 78 (1977): 471-490.3. Schröder, A. K. et al. Immunol 57 (1985): 305-309.4. Rosenberg, I.M. Protein Analysis and Purification, Birkhäuser, 2005: 41-47.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Protein A
Shipping Condition	Dry ice
Available
Manufacturer - Type	Labeled proteins
Manufacturer - Applications	Western Blotting; Immunoprecipitation (IP); Immunofluorescence (IF); Immunohistochemistry (IHC)
Manufacturer - Category	Dyes & Reagents Group 3 / Dyes & Reagents Gro / Labeling & detection / Proteins / Orange (565-620 nm)
Manufacturer - Targets	Protein A
Shipping Temperature	4 °C
Storage Conditions	Stable for 2-3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles.
Molecular Weight	~30000
Manufacturer - Research Area	Peptide & Protein Analysis / Labeling Reagents
Description	Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M). AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30, 000 Da compared to the most reported 42, 000 Da for the native Protein A that is found in Staphylococcus aureus. Recombinant Protein A conjugated with HiLyte™ Fluor-555 can be used for immunoglobulin detection in IHC and IF and for assay development. Orange fluorescence. Excitation/Emission wavelength= 553 nm/568 nm
Absorbtion (nm)	553
Emission (nm)	568
Colour	Orange
Concentration	1 mg/mL
Storage Buffer	10mM phosphate, 150mM sodium chloride, pH 7.2, 0.1% sodium azide as preservative
Detection Method	Fluorescent
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	12352202
GTIN	5400535130077

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72236-safety-datasheet.pdf