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Caspase 3 (Apopain) Substrate 1f, fluorogenic

Caspase 3 (Apopain) Substrate 1f, fluorogenic

Item no.	AS-25273-5
Manufacturer	AnaSpec
CASRN	201608-14-2
Amount	5 mg
Category	Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Cell Biology Cell Status Apoptosis Reagents & Chemicals Staining & Dyes Fluorescent Reagents
Type	Fluorescent dyes and probes
Format	Lyophilized
Specific against	other
Purity	Peak Area by HPLC ≥95%
Sequence	Ac-Asp-Glu-Val-Asp-AFC
Citations	Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63.
ECLASS 10.1	30196090
ECLASS 11.0	30196090
UNSPSC	12000000
Alias	Ac-DEVD-AFC
Shipping Condition	Room temperature
Available
Manufacturer - Applications	FRET; Protease activity
Manufacturer - Category	Catalog Peptides / Catalog Peptides Gr / Peptides / Labeling & detection / Green (500 -550 nm)
Manufacturer - Targets	Caspase, Caspase 3
Manufacturer - Conjugate / Tag	Fluorescent dyes: AFC
Shipping Temperature	RT
Storage Conditions	- 20 °C Protected from light
Molecular Weight	729.7
Manufacturer - Research Area	Cancer & Apoptosis; Cell Signaling / Apoptosis & Cell Death; Enzyme Substrates & Inhibitors
Description	Caspase-3 substrate with kcat/Km = 1.3 ×106 M-1s-1, Caspase-7 substrate with kcat/Km = 1.3 ×104 M-1s-1; AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.
Absorbtion (nm)	380
Emission (nm)	500
Sequence One-Letter Code	Ac-DEVD-AFC
Detection Method	Fluorescent
Feature	Fluorogenic substrates
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	12352202
GTIN	5400535038434

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-25273-5-safety-datasheet.pdf