Comparison

Calpain Substrate, Fluorogenic, (Z-AA)2Rh110 - 5 mg

Item no. AS-60320-5
Manufacturer AnaSpec
Amount 5 mg
Category
Type Fluorescent dyes and probes
Format Lyophilized
Specific against other
Conjugate/Tag Unconjugated
Purity Peak Area by HPLC ≥95%
Sequence (Z-Ala-Ala)2Rh110
Citations Gitler D and Spira ME (1998). Real time imaging of calcium-induced localized proteolytic activity after axotomy and its relation to growth cone formation. Neuron 20, 1123-35, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54, Rothe G, et al. (1992). Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 373, 547-54.
ECLASS 10.1 30196090
ECLASS 11.0 30196090
UNSPSC 12000000
Alias (Z-AA)2Rh110
Shipping Condition Room temperature
Available
Manufacturer - Type
Catalog
Manufacturer - Applications
Enzyme activity
Manufacturer - Category
Peptides
Manufacturer - Targets
Calpain
Shipping Temperature
RT
Storage Conditions
-20°C Protected from Light
Molecular Weight
885.6
Description
A very sensitive fluorogenic calpain substrate, Abs/Em=501/527 nm. In general, Rh110-based protease substrates are much more sensitive than the analogous AMC or AFC-based substrate. This enhanced sensitivity might be attributed to the greater fluorescence of the enzymatic product. The colorless and nonfluorescent (Z-Ala-Ala)2Rh110 is hydrolyzed to the highly fluorescent Rhodamine 110, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, (Z-Ala-Ala)2Rh110 can also be used to detect calpain in a chromogenic mode since the enzymatic product (Rhodamine 110) exhibits a large extinction coefficient (close to 100, 000 cm-1mol-1). After cleavage, Abs(max) is at 497 nm and Em(max) is at 527 nm.
Product Group
LB1A3Z
Research Area
Cell Signaling; Cancer & Apoptosis / Lyophilized
Solvent System
DMSO
Absorbtion (nm)
497
Emission (nm)
527
Sequence One-Letter Code
(Z-AA)2Rh110
GTIN
5400535158453
Detection Method
Fluorescent
Usage
Research use
UNSPSC
12352202

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 mg
Available: In stock
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