Streptavidin, HiLyte™ Fluor 750 conjugated - 1 mg

Immunohistochemistry (IHC); Immunofluorescence (IF); Fluorescence in situ hybridization (FISH); Flow Cytometry; Western Blotting

Streptavidin

Stable for 2-3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles.

HiLyte Fluor750-streptavidin conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. Spectrally similar to Cy7 dye, HiLyte Fluor750 is the longest-wavelength HiLyte Fluordye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte Fluor647 or allophycocyanin (APC), facilitating multicolor analysis. With a peak excitation at ca.754 nm, conjugates of HiLyte Fluor750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720-750 nm range. The fluorescence signal of HiLyte Fluor750-conjugate can be observed at 778 nm with the excitation at 754 nm.

Peptide & Protein Analysis / In solution

778

Orange

10 mM phosphate, 150 mM NaCl, pH 7.2, with BSA and 2 mM NaN3

Research use