Comparison

5(6)-FAM [5-(and-6)-Carboxyfluorescein] UltraPure Grade Mixed Isomers - 1 g

Item no. AS-81002-1
Manufacturer AnaSpec
Amount 1 g
Category
Type Fluorescent dyes and probes
Format Lyophilized
Specific against other
Purity Peak Area by HPLC ≥95%
Citations 1. Biswas S, et al. J Colloid Interface Sci 271, 157-62; (2004).2. Ma LY, et al. Spectrochim Acta A Mol Biomol Spectrosc 60, 1865-72; (2004).3. Kermis HR, et al. Analyst 128, 1181-6; (2003).4. Kermis HR, et al. Biotechnol Prog 18, 1047-53; (2002).5. Ozkan P, et al. R Biochim Biophys Acta 1572, 143-8; (2002).6. Marechal X, et al. Photochem. Photobiol 70, (5) 813-819; (1999).
ECLASS 10.1 30196090
ECLASS 11.0 30196090
UNSPSC 12000000
Alias [5-(and-6)-Carboxyfluorescein] *UltraPure Grade* *Mixed Isomers*
Shipping Condition Room temperature
Available
Manufacturer - Type
Fluorescent labeling dye
Manufacturer - Applications
Labeling
Manufacturer - Category
Labeling & detection
Shipping Temperature
RT
Storage Conditions
-20°C Protected from light
Molecular Weight
376.3
Description
Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
Product Group
PA2ZZZ
Research Area
Peptide & Protein Analysis / Lyophilized
Solvent System
DMF or DMSO
Absorbtion (nm)
494
Emission (nm)
519
GTIN
5400535129736
Colour
Green
Detection Method
Fluorescent
Usage
Research use
UNSPSC
12171501

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 g
Available: In stock
available

Delivery expected until 10/16/2025 

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