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Peroxidase-conjugated IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)

Peroxidase-conjugated IgG Fraction Monoclonal Mouse Anti-Human IgE (ME.114)

Item no.	209-032-241
Manufacturer	Jackson ImmunoResearch
Amount	0,5 ml
Category	Antibodies Secondary Antibodies
Type	Antibody Monoclonal
Format	Lyophilized
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Available
Manufacturer - Category	Anti-Human IgE Antibodies
Manufacturer - Conjugate / Tag	Horseradish Peroxidase
Storage Conditions	Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Expiration Date	one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Extended Storage after Rehydration	Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Preservative	None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Suggested Working Concentration or Dilution Range	1:5, 000 - 1:100, 000 for ELISA and Western blotting with chromogenic substrates 1:10, 000 - 1:200, 000 for Western blotting with ECL substrates Not recommended for detection of reduced IgE Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Buffer	0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer	15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Manufacturer - Specificity	IgE
Clonality	Monoclonal
Infomation	Based on immunoelectrophoresis and/or ELISA, the antibody reacts with human IgE. No antibody was detected against human IgG, IgM, or IgA. The antibody may cross-react with immunoglobulins from other species.
Physical State	Freeze-dried solid
Purity	The IgG fraction was purified by Protein A chromatography.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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209-032-241-safety-datasheet.pdf