Item no. |
B-BNC742905-500 |
Manufacturer |
Biotium
|
Amount |
500 uL |
Category |
|
Type |
Antibody Monoclonal |
Applications |
FC, IF, IP |
Clone |
12G10 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG1 Kappa |
Citations |
Note: References for this clone sold by other suppliers may be listed for expected applications. FEBS Lett (1995) 363: 118-122. (functional studies)J Cell Sci (2005) 118: 4009-4016. (IP)Traffic (2012) 13: 610-625. (Flow, IF) |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
Fibrinogen Receptor beta subunit; FNRB; Glycoprotein IIa (GPIIA); Integrin beta-1 (ITGB1); integrin VLA-4 beta subunit; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB |
Shipping condition |
Room temperature |
Available |
|
Manufacturer - Type |
Primary |
Manufacturer - Applications |
Flow, surface (published)|Functional studies (published)|IF (published)|IP (published) |
Manufacturer - Category |
Antibodies |
Manufacturer - Targets |
CD29 |
Manufacturer - Conjugate / Tag |
CF740 |
Shipping Temperature |
Room temperature |
Storage Conditions |
Store at 2 to 8°C|Protect fluorescent conjugates from light |
Molecular Weight |
110 kDa (non-reduced), 130 kDa (reduced) |
Description |
Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. |
Product origin |
Animal |
Ingredient of biological origin |
Mus musculus (mouse), Bos taurus (bovine) |
Special handling for PI and label |
Protect from light |
Undated stability guarantee in PI |
2 years |
Stability at RT during shipping (protected from light) |
Stable at room temperature or 37°C (98°F) for 7 days. |
UNSPSC Commodity |
41116161 |
UNSPSC Commodity Title |
Primary and secondary antibodies for multiple methodology immunostaining detection application |
Classified or regulated chemicals |
0.05% sodium azide (CAS 26628-22-8) |
Concentration |
0.1 mg/mL |
Storage buffer |
PBS, 0.1% rBSA, 0.05% azide |
Dated shelf life printed on label |
Guaranteed for at least 24 months from date of receipt when stored as recommended |
Immunogen (Secondaries, for anti tag only) |
Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract. |
Clonality |
Monoclonal |
Regulatory Status |
For research use only (RUO) |
Human Gene Symbol |
ITGB1 |
Unigene |
643813 |
Positive Control |
HT1080 whole cell lysate. HeLa cell line. |
Antibody target cellular localization |
Plasma membrane |
Cell tissue expression |
Platelets|Endothelial cells|Fibroblasts|Lymphocytes|Mast cells|Monocytes/macrophages|Smooth muscle |
Antibody application notes |
Does not react with Mouse or Rat; ELISA: For coating use Ab at 1-5 ug/mL, order Ab without BSA; Optimal dilution for a specific application should be determined by user.|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody |
Antibody Number |
#2905 |
Expected Antibody Applications |
Flow, surface (published for clone), Functional studies (published for clone), IF (published for clone), IP (published for clone) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.