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Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

Anti-Apoptosis repressor with CARD/NOL3 Antibody Picoband®

Item no.	BOS-PA1793-carrier-free
Manufacturer	Boster
Amount	100 ug
Quantity options	100 ug/vial 10 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug 100 ug
Category	Neuroscience Molecular Targets for Neuroscience Aging & Neurological Disorders Neural Signaling Antibodies Western Blot Cardiovascular Research Ischemia & Reperfusion Injury Primary Antibodies
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB
Specific against	Human (Homo sapiens), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<0.1ng/ml
Sequence	Contains 1 CARD domain.
Citations	1. Abmayr, S., Crawford, R. W., Chamberlain, J. S.Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle.Hum. Molec. Genet. 13: 213-221, 2004. 2. Koseki, T., Inohara, N., Chen, S., Nunez, G.ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases.Proc. Nat. Acad. Sci. 95: 5156-5160, 1998. 3. Stoss, O., Schwaiger, F.-W., Cooper, T. A., Stamm, S.Alternative splicing determines the intracellular localization of the novel nuclear protein Nop30 and its interaction with the splicing factor SRp30c.J. Biol. Chem. 274: 10951-10962, 1999.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Nucleolar protein 3,Apoptosis repressor with CARD,Muscle-enriched cytoplasmic protein,Myp,Nucleolar protein of 30 kDa,Nop30,NOL3,ARC,NOP
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Conjugate / Tag	carrier-free
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular weight	27 kDa
Calculated Molecular weight	23 kDa
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5μg/ml, Human, Rat
Gene Name	NOL3
Gene Full Name	Nucleolar protein 3
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	NOL3 (Nucleolar protein 3), also known as ARC, NOP30, CARD2 and MYP, is a protein that in humans is encoded by the NOL3 gene. NOL3 has been shown to interact with SFRS9 and Caspase 8. By genomic sequence analysis, Stoss et al. (1999) determined that the NOL3 gene, which encodes NOP30 and MYP and which they called NOP, is composed of 4 exons. The alternative 5-prime splice site that generates the 2 isoforms is located in exon 2. It is reported that expression of the ARC cDNA encoding the smaller transcript inhibited apoptosis in a dose-dependent manner when coexpressed with CASP8 but not when coexpressed with CASP9. ARC also inhibited apoptosis induced by stimulation of CD95/FAS, tumor necrosis factor receptor-1, and TRAMP/death receptor-3. Enzymatic analysis showed that ARC inhibits the enzymatic activity of CASP8. Immunoprecipitation and immunoblot analysis indicated that ARC interacts with CASP2 and CASP8 through its N-terminal death effector domain but does not interact with CASP1, CASP3, or CASP9.
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of human Apoptosis repressor with CARD, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Manufacturer - Research Category	Apoptosis, Caspases, Cell Biology, Intracellular
Protein Name	Nucleolar protein 3
Protein Function	Isoform 1: May be involved in RNA splicing.
Subcellular Localization	Isoform 1: Nucleus, nucleolus. The SR-rich C-terminus mediates nuclear localization.
Description	Boster Bio Anti-Apoptosis repressor with CARD/NOL3 Antibody catalog # PA1793. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Tissue Specificity	Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
Sequence Similarities	Contains 1 CARD domain.
Manufacturer - Cross Reactivity	No cross-reactivity with other proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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BOS-PA1793-carrier-free-datasheet.pdf

BOS-PA1793-carrier-free-safety-datasheet.pdf