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Anti-6xHis Antibody

Anti-6xHis Antibody

Item no.	75-169
Manufacturer	Antibodies Incorporated
Amount	100 uL
Category	Antibodies ELISA Immunocytochemistry Primary Antibodies Immunoblot
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IHC, ICC, ELISA
Clone	N144/14
Specific against	other
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Targets	6xHis
Country of Origin	United States
Shipping Temperature	Shipped on ice packs
Storage Conditions	Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Molecular Weight	1 kDa
Manufacturer - Research Area	Anti-Tag Antibodies
Expiration	24 months from date of receipt
Immunogen	Identified as off-target mAb in screen for anti-ProtocadherinGammaA3 Neuromabs (immunogen was 6xHis fusion protein amino acids 720-804, variable portion of cytoplasmic C-terminus, of mouse ProtocadherinGammaA3). Target identified as 6xHis by ELISA, immunofluorescence and immunoblot against other 6xHis-tagged fusion proteins.
Immunogen Species	Mouse
Description	Our Anti-6xHis mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N144/14. It detects 6xHis, and is purified by Protein A chromatography. It is great for use in ELISA, ICC, WB.
Target Description	6xHIS or His-tag is a commonly used recombinant tag that consists of 6 sequential histidine residues placed at the amino or carboxy terminus of a targeted sequence. 6xHis is useful for purification of proteins or visualization by western blot or immunocytochemistry.
Clonality	Monoclonal
Manufacturer - Specificity	No cross-reactivity reported
Concentration	1 mg/mL
Form	Purified by Protein A chromatography
Buffer	10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.96
Production Notes	Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Quality Control	Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
Quality Control: Application	ICC
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Manufacturer - Statement	We produce our 6xHis mouse monoclonal primary antibody from hybridoma clone N144/14. It is great in ELISA, ICC, WB and is purified by Protein A chromatography.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 uL

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Delivery expected until 9/11/2025

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