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Anti-Botch Antibody

Anti-Botch Antibody

Item no.	75-181
Manufacturer	Antibodies Incorporated
Amount	100 uL
Category	Antibodies Immunocytochemistry Primary Antibodies Immunoblot
Type	Antibody Monoclonal
Format	Liquid
Applications	WB, IHC, ICC
Clone	N116/14
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Glutathione-specific gamma-glutamylcyclotransferase 1 (Gamma-GCG 1) (EC 4.3.2.7) (Blocks Notch protein) (Botch) (Cation transport regulator-like protein 1) (Neuroprotective protein 7)
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Targets	Botch
Country of Origin	United States
Shipping Temperature	Shipped on ice packs
Storage Conditions	Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Molecular Weight	25 kDa
Manufacturer - Research Area	Neurogenesis and Development
Expiration	24 months from date of receipt
Immunogen	Fusion protein amino acids 1-222 (full-length) of rat Botch1 (accession number B3STU3.1) produced recombinantly in E. Coli
Immunogen Species	Rat
Description	Our Anti-Botch mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N116/14. It detects human, mouse, and rat Botch, and is purified by Protein A chromatography. It is great for use in ICC, WB.
Target Description	Cation transport regulator-like protein 1, Glutathione-Specific Gamma-Glutamylcyclotransferase 1 or Botch1 is encoded by the gene CHAC1. Chac1 is a member of the gamma-glutamylcyclotransferase family. It is an enzyme that acts to catalyze the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Chac1 is involved in neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. CHAC1 is expressed in tissues including brain (forebrain and anterior spinal cord), and heart. Diseases associated with CHAC1 include Ovarian Embryonal Carcinoma and Ovarian Cancer.
Clonality	Monoclonal
Manufacturer - Specificity	No cross-reactivity against Botch2/Chac2
Concentration	1 mg/mL
Form	Purified by Protein A chromatography
Buffer	10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.109
Production Notes	Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Quality Control	Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
Quality Control: Application	ICC
Dilution Range: WB	1:2000
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Manufacturer - Statement	We produce our Botch mouse monoclonal primary antibody from hybridoma clone N116/14. It is great in ICC, WB and is purified by Protein A chromatography.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 uL

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Delivery expected until 9/11/2025

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