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Anti-Histone H3.3 Antibody

Anti-Histone H3.3 Antibody

Item no.	75-228
Manufacturer	Antibodies Incorporated
Amount	100 uL
Category	Oncology Antibodies By Cell Type Blastoma Glioma Immunohistochemistry ELISA Primary Antibodies Astrocytoma
Type	Antibody Monoclonal
Format	Liquid
Applications	IHC, ELISA
Clone	N158/28
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Mouse
Isotype	IgG1
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Histone H3.3
Shipping Condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Targets	Histone H3.3
Country of Origin	United States
Shipping Temperature	Shipped on ice packs
Storage Conditions	Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Molecular Weight	15 kDa
Manufacturer - Research Area	Epigenetics
Expiration	24 months from date of receipt
Immunogen	Synthetic peptide amino acids 83-93 (LRFQSAAIGAL) of rat Histone H3.3 (accession number P84245)
Immunogen Species	Rat
Description	Our Anti-Histone H3.3 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N158/28. It detects human, mouse, and rat Histone H3.3, and is purified by Protein A chromatography. It is great for use in ELISA, IHC.
Target Description	H3 Histone Family Member 3B is encoded by the gene H3.3B. H3.3B is a member of the histone H3 family. H3.3B contains introns and its mRNA is polyadenylated. H3.3B is a variant histone H3, and is a replication-independent histone. H3.3B is deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. H3.3B is expressed in 9 organs with the highest expression in the spleen. Diseases associated with H3.3B include Chondroblastoma, Clear Cell Chondrosarcoma, Bone Giant Cell Sarcoma, Malignant Giant Cell Tumor, and brain stem cancer.
Clonality	Monoclonal
Manufacturer - Specificity	Does not cross-react with Histone H3.1
Concentration	1 mg/mL
Form	Purified by Protein A chromatography
Buffer	10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Production Notes	Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Quality Control	Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
Quality Control: Application	IHC
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Manufacturer - Statement	We produce our Histone H3.3 mouse monoclonal primary antibody from hybridoma clone N158/28. It is great in ELISA, IHC and is purified by Protein A chromatography.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 uL

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Delivery expected until 9/25/2025

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