FAK, phosphorylated (Tyr397) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397. Species sequence homology: human, xenopus, chicken, mouse (100%).

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.

Applications:
Suitable for use in Western Blot and Cell Function Assay. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 detected phospho-FAK (Tyr397) in 10ug of untransfected and Src-transfected RAT2 cell lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
Untransfected and Src-transfected RAT2 cell lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.