Mitofusin-1 (Fzo Homolog, Transmembrane GTPase MFN1, MFN1)

Antibodies / Antibodies-GTPase, Rab, Ras, Rho Proteins

-20°C

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Mitofusin-1.

Along with Mitofusin-2 (Mfn2), Mitofusin-1 (Mfn1) is a known protein mediator of mitochondrial fusion. Mitochondrial fusion regulates mitochondrial morphogensis and supports complementation between mitochondrial genomes in mammalian cells. Two Mfn1 transcripts are detected at similar levels in a variety of human tissues and are dramatically elevated in the heart. Human Mfn1 is known to be localized in the mitochondria. It is reported that point mutations in the conserved G1 and G2 region of the Mfn1 GTPase domain result in decreased formation and fragmentation of the mitochondria, respectively.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml detected Mitofusin-1 in 10ug HeLa cell lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.