Nestin (NES, Nbla00170) (PE)

Antibodies / Antibodies-Neurofilament

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.1, 15mg/ml BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).

Fusion protein.

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:5; 5ul labels 5x10e5 cells in 95ul PBS,
10ul labels 1x10e6 cells in 90ul PBS.
Optimal dilutions to be determined by the researcher.

Positive Control:
U251 cells

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.