Ki-67 (Antigen KI-67, MKI67, Ki67, Ki 67)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid, 0.03% sodium azide.

KLH-conjugated synthetic peptide corresponding to the sequence CEDLAGFKELFQTPG of human KI-67.

Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA:1:10, 000.
Western Blot: 1:500-1:2, 000.
Immunohistochemistry: 1:200-1:1.000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.