NEFL (Neurofilament light polypeptide)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, 0.05% sodium azide, 0.5% protein stabilizer.

Purified recombinant protein fragment of human NEFL expressed in E. coli.

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Applications:
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/ Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:200-1:400
ELISA: 1:10, 000
Western Blot: 1:500-1:2000
Immunohistochemistry (Paraffin): 1:200-1:1000
Immunofluorescence (IC): 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.