CCND1-Y226 (CCND1, BCL1, PRAD1, G1/S-specific cyclin-D1, B Cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene)

Antibodies / Antibodies-Cyclin

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

CCND1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-241 amino acids from the C-terminal region of human CCND1.

CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Applications:
Suitable for use in Western Blot, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.