Csk, CT (Csk, Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase MPK-2, p50CSK) (Azide free) (HRP)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

Synthetic peptide corresponding to a portion within aa377-404 from the C-terminal region of mouse Csk, conjugated to KLH.
Species Sequence Homology: bovine, chicken and rat

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. HRP conjugates are sensitive to light.

Note: Applications are based on unconjugated antibody.