DKC1, ID (DKC1, NOLA4, H/ACA ribonucleoprotein complex subunit 4, CBF5 homolog, Dyskerin, Nopp140-associated protein of 57kD, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

DKC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human DKC1.

DKC1 is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.