LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2)

Antibodies / Antibodies-Enzymes, Deacetylase

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species Sequence Homology: Mouse

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA
Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry: 1:50-100
Immunofluorescence: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.